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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in many tumors, which leads to chromosome instability, and therefore may well contribute to tumorigenesis. This protein was identified to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a critical purpose in advertising and marketing mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be described. Two additional splice variants have been reported but specific nucleotide sequence data will not be nonetheless offered.
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Blocking peptides are peptides that bind particularly for the concentrate on antibody and block antibody binding. These peptide typically includes the epitope acknowledged from the antibody.
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This guidebook outlines even further possible optimization of cellular and immunoassay parameters to be certain the very best effects are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress by S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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